FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988456
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52920.5353804317035862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG150.00578421554.99797412
ACGGGTG257.8573695E-443.9983816
ACGGCCC257.8573695E-443.9983828
GGGGTAC358.666136E-539.2862618
TGGTATC15850.038.35022
GGCTCAT300.001925840736.66531828
GTGGTAT16850.036.244791
ACTGACC350.004100097331.4274169
GATACCA4600.028.09679211
GTATCAA64500.028.0219361
GACAGAC507.0161116E-427.510121
GTCTATA507.024417E-427.5045532
ATGAGCC400.007872079527.50037818
TCAAGGG400.007872079527.50037824
CACGGCC400.00787402327.4989927
CACTGCT3650.027.1222916
TGCTTAA3600.026.7364819
TTCAGGG651.0875882E-425.3888192
TCCTATA1001.3072167E-724.7540972
AGAGTAC36300.024.39304411
Produced by FastQC (version 0.11.5)