FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300689
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.1322647259147151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65250.040.0663341
TGGTATC18250.037.9598772
TATCAAC76000.034.504172
ACGGTCG500.00278802634.0038268
GTGGTAT21000.032.995391
GGACGTA652.5518064E-432.6974076
CAACGCA83300.031.6858625
ATCAACG84350.031.290073
GGTATCA33900.030.9729271
TCAACGC87900.030.1237224
AACGCAG96500.027.6623466
CGCAGAG104250.024.9932178
AGCGTCA3500.024.2863373
ACGCAGA111000.023.8562877
CGACTTT7200.023.6117133
GCAGAGT113650.023.0756269
GTCGACT7600.022.9327031
TCGAGGG26200.022.712489
GGCGTTC950.00234228322.3709378
ATACGTG950.002342874622.3699675
Produced by FastQC (version 0.11.5)