FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306279
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.13302813753236276No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.1221448055504126No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.10380357276808226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21550.043.3893552
GTGGTAT22100.042.5073741
GTATCAA73250.039.1125831
TATCAAC84450.034.0216062
GGTATCA36450.032.6531221
ATCAACG94000.030.70083
CAACGCA95750.030.2284645
GTCGTAC1901.8189894E-1229.0839921
TCAACGC99400.029.0329484
AACGCAG110550.026.7209976
AGCGTCA4750.025.9484923
CGCAGAG123100.023.7190348
GCGTCAG5150.023.10784
GGGTACG2950.023.0548461
ACGCAGA127450.022.9094817
GCAGAGT134600.021.8819759
TCGAGGG31750.021.6855099
TTAAACG5250.021.45284522-23
AGTCGAG61800.021.3880027
GTCGAGG58050.021.3785868
Produced by FastQC (version 0.11.5)