FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306279
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44290.19204094560978963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24650.038.8313562
GTGGTAT25200.038.21541
CGTTCCG300.001925965236.66689719
TTAAACG4600.029.29366122
GTATCAA95700.027.6300931
GTCGTAC1850.025.2841341
TATCAAC107800.024.4692132
AGAGTAC53600.023.75378411
ATCAACG117400.022.2765463
CAACGCA118200.022.101075
GGTATCA44950.022.0977671
GACGCGA752.8733298E-422.00061431
TCAACGC119650.021.8107244
TCGTACC1801.0913936E-1121.39322
CAGAGTA60250.021.13199810
CGGGGAC1701.2551027E-1021.02908717
TGCGTTG8200.020.7914625
CGTACCC2001.8189894E-1220.625133
CGGGACG804.444604E-420.62468317
CGTCGAA804.4459043E-420.62378927
Produced by FastQC (version 0.11.5)