FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046629
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.1710252630110574No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.162712861959682No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14750.14092863851469814No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.1323296029443098No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT12330.11780678731432054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3600.031.8762533
CGACTTT3900.031.6038073
GTATCAA61800.031.0195561
GTCGACT4350.029.3142991
AACGCAG64300.028.754456
GCGTCAG4150.028.6758254
CTAACGC1054.522539E-628.3344483
TATCAAC69950.027.5850732
ATCAACG73500.026.1949063
TCAACGC75300.025.5122934
CAACGCA75500.025.4459255
CGCAGAG73850.025.0923888
ACGCAGA74800.024.716887
TATACGT1401.5307614E-624.286674
GCAGAGT77200.024.0035369
GTGGTAT25900.023.6325321
ATACGTG1302.3693341E-522.886615
GTATTAG4100.022.8079551
GTCGAGG34350.022.6428138
GTACTAG1703.2820935E-722.5030361
Produced by FastQC (version 0.11.5)