FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41460
Sequences flagged as poor quality0
Sequence length91
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT36018.685479980704294No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGG4961.1963338157260008No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT4371.0540279787747227TruSeq Adapter, Index 11 (95% over 23bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTA3690.890014471780029No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGGCGT3000.723589001447178No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT2830.6825856246985046No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC2320.5595754944524843No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA2300.5547515677761697No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGTCGTATGCCGT2110.5089242643511819No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA1950.47033285094066574No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAGCTCGTATGCCGT1850.44621321755909316No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA1830.44138929088277856No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT1820.4389773275446213RNA PCR Primer, Index 11 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG1780.42932947419199224No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA1690.4076218041485769No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCGT1670.4027978774722624Illumina PCR Primer Index 11 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1590.3835021707670043TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGCCGT1380.33285094066570187No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGGCGG1350.3256150506512301No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA1270.30631934394597204No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTACA1180.2846116739025567No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.2846116739025567No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTGTGCCGT1170.2821997105643994No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.2653159671972986No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACCTCTCGTATGCCGT1100.2653159671972986No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGGATGCCGT1060.25566811384466953No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGG1000.241196333815726No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGG970.23396044380125425No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA900.21707670043415342No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.20260492040520983No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT810.19536903039073808No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA800.1929570670525808No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT790.19054510371442354RNA PCR Primer, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTGTGCCGG780.18813314037626627No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT750.1808972503617945No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATTCCGT710.17124939700916547No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1688374336710082No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.16160154365653642No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA660.15918958031837918No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTGTT630.15195369030390737Illumina PCR Primer Index 11 (95% over 22bp)
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA620.1495417269657501No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAG590.14230583695127835No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGGCGT560.13506994693680657No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCGTCT550.1326579835986493Illumina PCR Primer Index 11 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTGGT540.13024602026049203No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGCCGG540.13024602026049203No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGTCGTATGCCGG510.12301013024602027No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCG500.120598166907863RNA PCR Primer, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTGTGGCGG500.120598166907863No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGGATGCCGG490.11818620356970574No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC490.11818620356970574No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA490.11818620356970574No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA490.11818620356970574No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATATCGTATGCCGT490.11818620356970574No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGGCGT480.11577424023154848No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.1133622768933912No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGGCGG470.1133622768933912No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTGT450.10853835021707671Illumina PCR Primer Index 11 (95% over 24bp)
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGGAGCTACAT450.10853835021707671No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGTCGTATGGCGT440.10612638687891944No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGGCGG440.10612638687891944No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT430.10371442354076219No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10371442354076219No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCGTGG420.10130246020260492Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)