FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41460
Sequences flagged as poor quality0
Sequence length61
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1660740.05547515677762No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1630.3931500241196334No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA670.16160154365653642No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA660.15918958031837918No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.1447178002894356No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA600.1447178002894356No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAAG150.005744500655.053
GTGGTAT850.045.2941171
TGGTATC800.044.6874962
AGATGGA453.7333686E-430.5555531
TGGATTG553.4111916E-530.04
GGTATCA1251.8189894E-1228.5999983
GGATTGG1203.274181E-1127.51
TGGGGTT1303.6379788E-1227.4999985
GATTGGG1255.638867E-1126.42
TTGGGGT1255.638867E-1126.44
TGGGACC759.459987E-625.6666665
ATTGGGG1309.094947E-1125.3846133
GTATCAA2400.025.2083341
TTGGGAC701.7601969E-423.5714284
GGGGTTG1653.6379788E-1223.3333346
CGCTGCC600.00200281522.91666655
GATGGAT600.00200281522.9166662
GGGACCA852.493966E-522.6470586
TATCAAC2600.022.2115362
GGACCAT903.8774895E-521.3888877
Produced by FastQC (version 0.11.5)