FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725863
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44510.2578999607732479No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.19277312277973396No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.15487903732799185No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22840.13233958894767428No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18960.10985808259404135No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT17630.10215179304498678No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA17270.10006588008434042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82950.044.6840971
TGGTATC18650.044.211782
CTCACGG801.1290467E-842.50389
GTGGTAT20450.041.1552051
TATCAAC99950.037.2083472
GGTATCA40550.036.1643871
ATCAACG111500.033.5827453
CAACGCA114800.032.692385
TCAACGC114700.032.6467674
ATACGTG550.004446676530.9118525
AACGCAG124300.030.2288366
CGACTTT7350.030.0698413
CCTCACG1152.7481474E-729.5678588
GTCGACT7950.027.8028351
CGCAGAG134550.027.7988418
GCAGAGT142750.026.261549
ACGCAGA144900.025.929797
GTATCAC1159.167423E-625.8733731
AGGGTAG1159.169486E-625.8726236
TGTAGCG1201.2758033E-524.7938795
Produced by FastQC (version 0.11.5)