FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187776
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.44734151329243355No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.39728186775732793No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.39461912065439675No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT6080.3237900477164281No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.3237900477164281No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.2716002044989775No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4800.2556237218813906No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.23911468984321743No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.19171779141104295No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.17148091342876617No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.15390678254942058No Hit
CTGTGAGACAACACAAAATTAAAATTCAGGAAGCTGTTTGAATCATTAGT2810.14964638718473075No Hit
TTCTGGAGGAGAGTAAAAAACAAAAGAAGACAGTGAAGAATGAGGAATGT2720.14485344239945466No Hit
GCCTAGAATAGTGCCTGGCACATGATTAGGATTTAATTATTACTTCTGAA2660.14165814587593728No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.13739775051124745No Hit
ATGCAGCACAGACCAATTAGCCAAATGCAAAATAAACTAGATTCTTACCA2390.12727931152010905No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.12461656441717792No Hit
GTATATAACCATGCAGCACAGACCAATTAGCCAAATGCAAAATAAACTAG2300.12248636673483299No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.11929107021131562No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.11875852079072938No Hit
ATTCTAGGCACTAGAAAAAAAAACCAGCAGTGAACAGAAAACAGATGAAA2210.11769342194955693No Hit
CATATATATAGACAATTTACAAAATAGCAGAATTTCATAAGCATTCTGGA2200.1171608725289707No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG2180.11609577368779822No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.11609577368779822No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG2140.1139655760054533No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT2120.11290047716428085No Hit
GTTCCTGACGGAGCTGACCAGACTTTTCCAGAAGTGCCGGACGTCGGGCA2080.11077027948193593No Hit
GCCTAGTACAAAGTCCAAATTCTTACTTTATGGATGTAAAATGTCCAGGT2050.10917263122017723No Hit
CCTATAAACACTGCAACTATTCTTTCCAAAAGGACCCTAATCTTATGTGA2040.108640081799591No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.108640081799591No Hit
GTCTGAGCATGGCTGAAAACCGTGGTGATCTTGTAGCAACCTGGACATTT2030.10810753237900476No Hit
GTTCTAGAGGTACTTATACACAACCTTTGAGGGCACAGTTTGGAGGTGGA1980.10544478527607362No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.1049122358554874No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA200.003212570763.7835731
GCGTATC200.003212570763.7835731
ATAGGCT401.4535963E-553.124674
AAACTCT250.00778635150.999686
AGCGTCA250.00778635150.999683
GGAAACT250.00778635150.999684
GCGTCAG250.00778635150.999684
CAATACC250.00778635150.999684
ATATAGG452.917388E-547.2219282
TCATGGA452.917388E-547.2219282
TATAGGC452.917388E-547.2219283
AGGCTGA452.917388E-547.2219286
GTATCAA23650.038.8365061
GGCCATG559.5316616E-538.636129
GAGGCCA559.5316616E-538.636127
CATAGGA805.3808617E-737.1872672
CATGGAG601.5905159E-435.4164433
TCATAGG858.6128966E-735.0184331
TGGTATC6950.034.8558962
TATCAAC27900.033.8169942
Produced by FastQC (version 0.11.5)