FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187776
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30431.620547886843899No Hit
GTCTGAGCATGGCTGAAAACCGTGGTGATCTTGTAGCAACCTGGACATTTTACATCCATAA2220.11822597137014315No Hit
CTGTGAGACAACACAAAATTAAAATTCAGGAAGCTGTTTGAATCATTAGTGTTGCTTTCTT2140.1139655760054533No Hit
ATGCAGCACAGACCAATTAGCCAAATGCAAAATAAACTAGATTCTTACCACAACTATCCTA2140.1139655760054533No Hit
ATTCTAGGCACTAGAAAAAAAAACCAGCAGTGAACAGAAAACAGATGAAAATCTCTGATCT2120.11290047716428085No Hit
GTATATAACCATGCAGCACAGACCAATTAGCCAAATGCAAAATAAACTAGATTCTTACCAC2080.11077027948193593No Hit
GCTGTATACCTTACAAAACCAAATGTAATTACATTATCTTGGTAAATTAATTAGAATTATG2000.10650988411724609No Hit
TTCTGGAGGAGAGTAAAAAACAAAAGAAGACAGTGAAGAATGAGGAATGTGGCAATTTTAA1920.10224948875255625No Hit
GCCTAGAATAGTGCCTGGCACATGATTAGGATTTAATTATTACTTCTGAATAAAGGTATTT1910.10171693933197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT150.005776879454.9981417
AGTACGT150.005776879454.9981413
TACGTGG150.005776879454.9981415
ACGGGCT150.005776879454.9981416
GAAGGTC150.005776879454.998144
GAATCAA257.789645E-444.057171
TACTGGG358.641756E-539.2843815
GAGGCCA300.001921847536.6654247
AGGGTTG300.001921847536.66542418
GAGTACT458.80687E-636.66542412
TCTGATC704.0472514E-935.3559454
TGGTATC8350.032.2915532
GTGGTAT8350.031.987621
TCATGGA350.004081000631.4442562
AGGCCAT350.004091661431.4275058
ACGGGAT350.004091661431.42750516
CCACTGC453.7843265E-430.5545215
CCCCTCA453.7843265E-430.5545244
TCCCCTC453.7843265E-430.5545243
AGCAAGA553.4636465E-530.014972
Produced by FastQC (version 0.11.5)