FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859735
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55080.29617122869656165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25450.039.362771
CGACACT1000.035.74909214
TGGTATC28950.034.7781142
GCGACCG501.8205785E-533.0000518
CGACCGT601.7179482E-632.0833819
TGGCGTA601.7183083E-632.0825236
TAGACGG350.004100790231.4277745
GTCTAGA1103.929017E-1027.5215021
GGACGTA705.709355E-627.5000419
GTATCAA108400.026.5821131
GTTCGGT858.7982335E-725.88239120
AACTACG759.752481E-625.6680857
CCGCTCG1201.0877557E-925.2083721
CTAACGA550.00122663725.000718
AGAGTAC57300.024.66844711
CAGAGTA60500.023.72730810
TATCAAC122250.023.4922772
GGTATCA52000.023.2344971
TTCGGTC952.317236E-623.15792821
GCATCCG852.573001E-522.64526439
Produced by FastQC (version 0.11.5)