FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808498
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.13685389754370753No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.12607146925238513No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18310.10124423693031455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16000.043.5657041
TGGTATC18100.039.448232
GTATCAA62900.035.6107251
GGTATCA35750.032.575821
TATCAAC73550.031.3194032
TATAGCG353.9451176E-430.35685570-71
ATCAACG80200.028.881453
TCAACGC80400.028.5981564
CAACGCA81700.028.4039945
TCGAGGG25200.027.6599989
TAGTGCG1405.028778E-827.3226819
AGCGTCA2650.027.2646453
AACGCAG88000.026.5161536
GCGTCAG2800.025.8040394
CGTTGAT11650.025.5376537
GCGTTGA11750.025.321016
TGCGTTG11700.024.7019865
GTCGACT5900.024.4933241
CGACTTT5800.024.1814733
GTCGAGG49450.023.2063228
Produced by FastQC (version 0.11.5)