FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085949
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.41309490593020487No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.3027766497321698No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.26778421454414525No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.21768978101181546No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT19180.17661971234376567No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA17590.16197814077825018No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT16510.15203292235639057No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA15380.1416272771557412No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.12486774240779264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18650.048.3258861
TGGTATC19100.047.1742862
GTATCAA74700.044.277371
CATAAGC951.1459633E-940.2644423
TATCAAC86100.038.7004242
GGTATCA32500.038.3271981
GTGATCG450.001662745337.7789849
CTGACGT450.001662745337.7789849
GCATGCA903.1903255E-837.7789848
CTAGGCA2150.035.582534
ATCAACG95650.034.9697573
CGTACGG500.00278848234.0010872
CAACGCA99100.033.7967835
TCAACGC99250.033.615684
TATAGTG2050.033.1717952
AACGCAG110600.030.513236
TATACGT2700.029.9083634
TAGTCCG1003.0923184E-629.7523215
CCTTAGG1003.093428E-629.7509522
AGCATGC1152.747929E-729.5661647
Produced by FastQC (version 0.11.5)