FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085949
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74710.6879696928677129No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12580.11584337754351265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA257.85804E-443.998014
GTGGTAT23400.040.9370571
TGGTATC23300.040.61962
ACCCCTA651.9790605E-938.07695848
CGGGGAC1300.033.84462417
TACGCGG758.010829E-932.9985153
CTACGCG758.010829E-932.9985152
GTACGTG501.8204666E-532.99850514
ACCTCGC350.004100442431.4271538
GTATCAA103550.029.8526481
CGACCCC904.719186E-827.50002346
CGTACCC507.0316356E-427.50002133
GAGTACT904.721187E-827.49875612
GTCCGCT805.1810275E-727.4987567
AACCGGC1004.316462E-927.49875526
AGAGTAC50900.027.4447311
CAGAGTA52600.026.97596710
TATCAAC116950.026.3246732
TAGTCCG957.9746314E-826.0514555
GGTATCA45300.025.8859791
Produced by FastQC (version 0.11.5)