FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875911
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35240.40232398040440187No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA19450.22205452380435914No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC17760.20276032610619116No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA17600.2009336565016309No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT15110.17250611078066153No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA14700.16782526991897578No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA14560.16622693401498553No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGT11340.1294652082232099No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG10490.11976102594898339No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT10380.11850519059584821No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA10310.11770602264385309No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT10220.11667852099128792No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAAT10170.11610768673986284No Hit
ATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA9950.11359601603359246No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTT9710.11085601162675203No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA9370.10697433871706143No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCC9000.10275016525651579No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCT8830.1008093288016705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG150.00578327254.99971453
CGGAGTC150.00578457854.9965810
CGAATTG257.857863E-443.9972629
CGGTCAC504.1176827E-738.50639730
TGGTATC12150.038.257382
GTGGTAT13100.036.9632531
CCGCAGC300.001923803636.67275632
TTTCGGA300.001925961936.66438710
TCGGAGC300.001925961936.66438712
TTCGGAG300.001925961936.66438711
GACCGGT401.8993225E-434.3807133
CTGCGGA1050.034.0454989
AGTAAGG501.8202783E-532.99794813
ACTGCGG1100.032.497988
GCGTTCT350.004099210331.4284124
CGAAGTA350.004099210331.4284124
GCTGCGC1057.2759576E-1231.42840820
CGAGGTA350.00410035631.42661516
GCTAATG453.7911232E-430.56063330
ACGTGAA453.796201E-430.55365414
Produced by FastQC (version 0.11.5)