FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795501
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67860.8530473248933692No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA10540.13249511942788256No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA8790.11049640415285461No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT8220.10333110832041695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGG150.005786046454.9925816
CTCACTC709.458745E-1139.285353
GCGTAAA300.001924759736.66863614
AACCGGG300.001926542636.6617215
GTGGTAT13350.034.621661
CGAGGCA401.9004208E-434.3768469
TCACCGG350.004097805431.43025811
GTCGTCA350.004097805431.43025824
CGAATAA701.6070771E-731.43025826
CGTACAA350.004097805431.43025812
AGTACGT350.004097805431.43025813
GCGTACA350.004097805431.43025811
GTACGTG350.004097805431.43025814
CTGTGCG350.004097805431.4302589
CGGGATA701.6088416E-731.4263117
GACGAGG453.7961063E-430.5533567
TGGTATC15700.029.0779952
TAGGAAC952.4938345E-928.94710436
GTATCAA63500.028.2483921
CGCTGCT507.027797E-427.5014789
Produced by FastQC (version 0.11.5)