FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339794
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70942.0877355103386166No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18050.531204200191881No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7300.2148360477230322No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.16892587862057598No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.13949628304207845No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.12890162863381932No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGCA150.00578158854.9964646
TGCTTAA903.8198777E-1133.613919
TTAAACG757.967174E-933.00273522
CGCTCGA453.78813E-430.55808822
ACGGGAA1201.8189894E-1229.78975316
GACAGTA400.00786792427.4982347
CTGAGCG400.00786792427.4982349
ACGGGCC1203.6379788E-1127.49823216
AGAGTAC28100.026.12821611
CAGAGTA28700.025.48616610
GTGTATC651.0796383E-425.409161
GCCGCTC651.0861415E-425.38672320
GCCGGGG550.001224184225.00207354
GCTGACG550.001224184225.00207355
GCTCGAG550.001224184225.00207323
CGACTTT3350.024.6289083
CGGGGAA1800.024.44287517
ACTGCTT1251.7353159E-924.19844817
GTAGCAT801.6028742E-524.0644973
TCGACTT3450.023.922072
Produced by FastQC (version 0.11.5)