FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419680
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.10340210275738941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16650.041.106581
GTATCAA58150.035.309951
TGGTATC19700.034.9516532
TATCAAC63150.032.575492
ATCAACG63600.032.411833
CAACGCA65750.031.4166165
AACGCAG65650.031.2715426
TCAACGC67450.030.6878134
GGTATCA31400.026.80621
CGACTTT6200.026.7357163
ACGCAGA77050.026.5873687
TATACCG850.00122645325.0017265
GTCGACT6900.024.6439651
CGCAGAG86050.023.9053698
GCAGAGT90500.022.8238379
GCGTCAG3300.021.895454
CTGACGT1604.884132E-621.2514674
TCGAGGG17750.020.8315649
CGTCAGA3700.019.5283745
AGAGTAC41050.019.25753810-11
Produced by FastQC (version 0.11.5)