FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419680
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58980.24375123983336638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18300.036.847941
TGGTATC20300.032.923962
GTATCAA75100.026.0572091
ATAGGCG801.6095188E-524.0618573
GCCGTCC801.6095188E-524.06185737
TATCAAC82400.023.1984772
GGTATCA36700.022.9484631
AGAGTAC41050.022.30756611
ATCAACG87100.021.911013
TCAACGC88400.021.4634724
CAACGCA88600.021.3529515
ATGACCG1559.0221874E-1021.29019434
AACGCAG90050.021.2839476
CGCGTAG650.00324049821.15415622
GTCGGTA650.00324049821.15415624
AGTACGG43450.020.75894213
CAGAGTA45050.020.6320810
CCGTTGT804.4462705E-420.62359827
GCTTACG956.0643906E-520.2626179
TCGGTAG1108.2593415E-619.99946813
Produced by FastQC (version 0.11.5)