FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294553
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39030.17009848977121034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23000.036.01561
TGGTATC24450.033.7516942
CGCGATA400.007875808527.49923714
GTATCAA84200.025.9840621
CGGACGT651.0896317E-425.38391146
TTAAACG3400.023.45574422
TATCAAC93450.023.2541752
AGAGTAC41000.022.87181711
ATCAACG99100.021.5341453
CAACGCA100150.021.170625
TCAACGC100450.021.0800154
GGTATCA43300.020.8467311
ACGGGTA1207.601102E-720.6244316
GGACGTC804.4449663E-420.62442847
TTCGCAC804.4449663E-420.62442831
CAGAGTA46850.020.60286510
CGACGGA956.0632483E-520.26303718
AACGCAG106850.020.0490156
CGTTTGG1251.124401E-619.79988310
GCGCTAG700.004977688719.6427411
Produced by FastQC (version 0.11.5)