FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192349
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT5160.26826237724136853No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.18819957473134769No Hit
GCAGTGGGACTGAGCAAAGTGCTCATTCGAAAACAGCCTGCCTTGGAGAA2930.15232728010023447No Hit
AGGTGAGACTGTCCAATAATTCAGCTTTTTTCAAGGCATAGACCCAAGAC2500.1299720819967871No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.11957431543704414No Hit
CCTCATGAGTATCCCATAGCACAAACGGGCAAACTCTGTTACTGGCACAT2230.11593509714113409No Hit
GTCTCACCTCCTGCCATAGGGTCCTGAATGTTTCAGACCACAAGGGGCTC2220.11541520881314694No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC2140.11125610218924975No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10969643720528831No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA2070.1076168838933397No Hit
ACTCTGTCTTGGGCGTTTGAACTCAGGGAGGGAGGCCCTTGGGAGCAGGT2010.10449755392541683No Hit
CAGTGGGACTGAGCAAAGTGCTCATTCGAAAACAGCCTGCCTTGGAGAAG2010.10449755392541683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAG302.6398266E-670.8331153
ATCTAAG200.003219439663.7498052
CTGCAGC356.5900604E-660.7141044
TGGTATC1000.059.499822
GTGGTAT1050.056.681231
TTCTGGT608.785719E-1056.6664922
CATCTAA250.00777853351.013111
GCAGCGT250.00777853351.013111
TTATCAT250.00778653650.9998444
GGTGTTC703.441528E-948.571286
AATCTGC452.913116E-547.234361
TGCTATA452.917599E-547.222082
GTATCAA6200.046.6248861
CAGCGCC505.4350872E-542.499877
TCGGCGT255.4452805E-542.4998752-53
GATTGGG505.4350872E-542.499872
GTAAATC409.3001657E-442.499873
TATCAAC6950.041.5826072
CTGGTGT851.9141225E-839.9998784
TCTGGTG851.9141225E-839.9998783
Produced by FastQC (version 0.11.5)