FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192349
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82974.313513457309369No Hit
GCAGTGGGACTGAGCAAAGTGCTCATTCGAAAACAGCCTGCCTTGGAGAAGAAACCAGAGA2360.12269364540496701No Hit
TTCGAAAACAGCCTGCCTTGGAGAAGAAACCAGAGAGAAGGTTTGAGAAGGTTTCAAAGTC1950.10137822395749393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATGA150.005776641454.9992182
GTGGTAT1450.041.7561151
GATACCA300.001921777436.66614511
TGGTATC1700.035.587732
CGGAACA401.895838E-434.3745117
TGAATTC401.895838E-434.374515
CTACAGA658.3329724E-833.8456734
CTCAAAG601.7075799E-632.0828846
AATCAAC350.00409151231.4281232
TGCGGTC350.00409151231.42812351
GCCTGAT350.00409151231.42812346
GTGCTTC350.00409151231.42812349
AGGGGTC350.00409151231.42812310
CGGTCAT350.00409151231.42812353
CTGATGT350.00409151231.42812348
CCTGATG350.00409151231.42812347
GTAAATC350.00409151231.4281233
GTATCAA7650.030.5789761
CCGGGCT453.7842267E-430.55512210
CTGGTGT453.7842267E-430.5551224
Produced by FastQC (version 0.11.5)