EGAF00005411370 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411370
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20868
Sequences flagged as poor quality	0
Sequence length	91
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA	317	1.5190722637531149	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT	290	1.3896875599003258	No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA	221	1.0590377611654207	No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA	197	0.9440291355184972	No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	168	0.8050603795284648	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.7811002491853556	No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA	134	0.642131493195323	No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA	128	0.6133793367835921	No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.5462909718228868	No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA	107	0.512746789342534	No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT	97	0.4648265286563159	No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCC	97	0.4648265286563159	No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.3929461376269887	No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA	77	0.36898600728387965	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGG	75	0.359401955146636	No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72	0.3450258769407705	No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA	67	0.3210657465976615	No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.30189764232317423	No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA	62	0.2971056162545524	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT	60	0.28752156411730884	No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.282729538048687	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGT	48	0.23001725129384704	No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47	0.22522522522522523	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	43	0.20605712095073794	No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC	43	0.20605712095073794	No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	43	0.20605712095073794	No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA	43	0.20605712095073794	No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42	0.2012650948821162	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGGGGGTGGCGG	40	0.19168104274487255	No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC	40	0.19168104274487255	No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG	40	0.19168104274487255	No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.19168104274487255	No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA	39	0.1868890166762507	No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT	39	0.1868890166762507	No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA	37	0.1773049645390071	No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA	37	0.1773049645390071	No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.1773049645390071	No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA	37	0.1773049645390071	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGG	37	0.1773049645390071	No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA	33	0.15813686026451984	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGG	33	0.15813686026451984	No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT	33	0.15813686026451984	No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA	30	0.14376078205865442	No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA	29	0.13896875599003258	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA	29	0.13896875599003258	No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCG	29	0.13896875599003258	No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTA	29	0.13896875599003258	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	27	0.12938470385278894	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGGCGG	27	0.12938470385278894	No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA	27	0.12938470385278894	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	26	0.12459267778416716	No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.11500862564692352	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGGTGGCGG	24	0.11500862564692352	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	24	0.11500862564692352	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAGCTCGTATGCCGT	23	0.1102165995783017	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGGATGCCGT	23	0.1102165995783017	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTGTGGCGG	23	0.1102165995783017	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC	22	0.10542457350967989	No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	22	0.10542457350967989	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTTTGCCGT	22	0.10542457350967989	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGATGGCGG	21	0.1006325474410581	No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.1006325474410581	No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA	21	0.1006325474410581	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACTT	45	0.0	85.00001	2
GACTTTT	45	0.0	85.00001	4
CGACTTT	45	0.0	85.00001	3
TGGTATC	20	0.0031792547	63.75	2
GGTATCA	75	0.0	62.333332	1
CCGCGAG	55	3.765308E-10	61.81818	1
GTCGACT	65	2.5465852E-11	58.846153	1
ACTTTTT	65	2.5465852E-11	58.846153	5
CGCGAGA	60	8.1490725E-10	56.666664	2
CGAGATG	55	2.669185E-8	54.090904	4
GCGAGAT	55	2.669185E-8	54.090904	3
GTGGTAT	25	0.0076899547	51.0	1
CTTTTTT	80	1.9099389E-10	47.8125	6
CGTTTGC	25	5.3617827E-5	42.5	40-41
TTGGGAC	80	1.0406438E-8	42.5	4
ACCATGC	85	1.7771526E-8	40.0	9
GTATCAA	160	0.0	39.84375	1
GTGTATA	55	9.242854E-5	38.63636	1
TGTATAA	55	9.242854E-5	38.63636	2
CTTATAC	725	0.0	38.103447	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content