FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11843
Sequences flagged as poor quality0
Sequence length91
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT3102.6175800050662836No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGG870.7346111627121507No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCC800.6755045174364603No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA590.4981845816093895No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGT590.4981845816093895No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT520.43907793633369924No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC420.3546398716541417No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG390.3293084522502744No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA390.3293084522502744No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGG370.31242083931436293No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGGGGGTGGCGG360.30397703284640715No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGGTGGCGG330.2786456134425399No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGGATGCCGT300.25331419403867267No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.24487038757071686No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGG270.22798277463480535No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA270.22798277463480535No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGGATGCCGG250.21109516169889384No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGGCGG250.21109516169889384No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTTTGCCGT250.21109516169889384No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTGTGGCGG240.2026513552309381No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTGTGCCGT230.19420754876298235No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTGTGGCGG230.19420754876298235No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGGGTGTGGCGG220.1857637422950266No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA220.1857637422950266No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCG210.17731993582707084No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAGCTCGTATGCCGT200.16887612935911508No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.16043232289115933No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTGTGCCGG180.15198851642320357No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGGCGT180.15198851642320357No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT180.15198851642320357No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT170.14354470995524782Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTGGTTTGGCGG160.1351009034872921No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTGGTATGGCGG160.1351009034872921No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA160.1351009034872921No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGGGTGGCGG150.12665709701933633No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA150.12665709701933633No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGATGGCGG150.12665709701933633No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTGGTATGCCGT140.11821329055138056No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTTTGCCGG140.11821329055138056No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTGTGGCGT140.11821329055138056No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTGGGGTGGCGG130.10976948408342481No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTGGTATGGCGT130.10976948408342481No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTTTGGCGG130.10976948408342481No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGGGGGTGGGGG120.10132567761546905No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA120.10132567761546905No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTGTGCCGG120.10132567761546905No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGTTGGCGG120.10132567761546905No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCGT120.10132567761546905No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA120.10132567761546905No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGGATGGCGG120.10132567761546905No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGGGGTTGGCGG120.10132567761546905No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGGATGCCGG120.10132567761546905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)