FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11843
Sequences flagged as poor quality0
Sequence length61
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG449937.98868529933294No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA230.19420754876298235No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.17731993582707084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.12665709701933633No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGG150.00564328155.050
CGAGGTT300.00184951636.66666816
AGGTTTT300.00184951636.66666818
TCGAGGT300.00184951636.66666815
GAGGTTT300.00184951636.66666817
GTATCAA401.790254E-434.3751
AACGCAG501.6728736E-533.06
TCAACGC453.5747685E-430.5555534
TATCAAC453.5747685E-430.5555532
ATCAACG453.5747685E-430.5555533
CAACGCA453.5747685E-430.5555535
ACGCAGA804.0942276E-420.6257
AGAGTCG804.0942276E-420.62511
CGCAGAG804.0942276E-420.6258
CAGAGTC856.162978E-419.41176410
GCAGAGT856.162978E-419.4117649
GGTTTTT750.006996562218.33333219
GAGTCGA909.0546743E-418.33333212
AGTCGAG750.006996562218.3333327
GTTTTTT1000.0018357516.520
Produced by FastQC (version 0.11.5)