FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237803
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.38729536633263667No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.2821663309546137No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.22876078098257804No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.2039503286333646No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA4080.1715705857369335No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4030.16946800502937306No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3280.13792929441596616No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3150.13246258457630897No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2900.12194968103850666No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2890.12152916489699458No Hit
GTTTAGAAGATGACAGGGCTCTCGAAGTCTCACCTGTCTTTTGTTTCTTT2850.11984710033094621No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.11900606804792202No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT2800.11774451962338574No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.11311884206675273No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT2590.10891368065163182No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2470.10386748695348672No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.10344697081197461No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2410.10134439010441416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA150.001028619984.998533
ATTAGGC150.001028619984.998533
ATTAGTC357.738345E-872.855873
TAGTCTT357.738345E-872.855875
ATGCTTT200.00321787763.76237
CAGCGTC200.0032205663.7488942
GAATTAG200.0032205663.7488941
ACTCAGG200.0032205663.7488941
ATCCATG356.5954064E-660.713231
TATTAGT454.4029184E-756.6656842
CCATGAT401.4548763E-553.1240773
CTTGCTA509.098203E-751.0098429
AGTCTTG509.098203E-751.0098426
GTCTTGC509.098203E-751.0098427
GTCAGAT354.8196322E-448.5705832
TCTTGCT551.7548846E-646.372588
TTATTAG551.7574293E-646.362831
GTGGTAT4150.044.035381
TGGTATC4350.042.987762
CATGATT505.4394448E-542.4992644
Produced by FastQC (version 0.11.5)