FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43601
Sequences flagged as poor quality0
Sequence length91
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA5501.2614389578220684No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5361.2293296025320521No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA4180.9586936079447719No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.9265842526547555No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA3660.83943028829614No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.7064058163803583No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.6765899864682002No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.5687942937088599No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT2350.5389784637967019No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA2200.5045755831288273No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA2170.4976950069952524No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT2040.4678791770830944No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1820.41742161877021167No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA1750.4013669411252035No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.389899314235912No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.32338707827802116No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.32338707827802116No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA1390.3188000275223045No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA1310.3004518244994381No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.2935712483658632No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA1220.2798100960987133No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC1170.2683424692094218No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1100.25228779156441367No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.24540721543083877No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.24082016467512213No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT960.22017843627439737No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG940.2155913855186808No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA900.20641728400724754No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA890.20412375862938922No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG810.18577555560652279No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC770.17660145409508957No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA750.17201440333937296No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.16972087796151464No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGG730.16742735258365632No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT690.15825325107222313No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA670.15366620031650652No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.15366620031650652No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCC610.13990504804935666No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.13990504804935666No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA600.13761152267149837No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA590.13531799729364005No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.13531799729364005No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA580.13302447191578173No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG580.13302447191578173No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC570.13073094653792344No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.13073094653792344No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT550.12614389578220683No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA550.12614389578220683No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG540.12385037040434851No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.12385037040434851No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA500.11467626889291531No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA480.11008921813719869No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA480.11008921813719869No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA470.1077956927593404No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT460.10550216738148208No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.10550216738148208No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.10320864200362377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGT302.2311635E-456.666677
TAACAAT302.2311635E-456.666671
ACGGTCC302.2311635E-456.666679
AGAGATT250.00774633851.08
TGGTATC1450.049.8275832
CAATAAC354.7757273E-448.5714264
AACGGTC354.7757273E-448.5714268
TCGACTT756.144546E-945.3333322
GTCGACT756.144546E-945.3333321
GTGGTAT1600.045.156251
ATGGCGT255.410477E-542.544-45
TGTATAA701.8434912E-742.52
AATAACG409.2209224E-442.55
GGTATCA3900.041.410251
CGACTTT851.8562787E-840.03
GAGCGCC753.1684976E-739.6666641
GCCGCGA753.1684976E-739.6666645
GTATCAA7200.037.777781
ATAACGG450.001645558337.777786
AGCGCCG805.255224E-737.18752
Produced by FastQC (version 0.11.5)