FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43601
Sequences flagged as poor quality0
Sequence length61
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32477.447076901905919No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA4000.9174101511433225No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.32797412903373774No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA1300.29815829912157976No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA1300.29815829912157976No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA1190.2729295199651384No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA790.18118850485080618No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA790.18118850485080618No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA720.16513382720579803No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.16284030182793974No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.15366620031650652No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA650.14907914956078988No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.13531799729364005No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA530.12155684502649022No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG520.11926331964863192No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1169697942707736No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1077956927593404No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.10550216738148208No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG440.10091511662576545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2300.034.673192
GTGGTAT2350.033.9744221
GTATCAA8950.028.607631
TCGACTT606.1825354E-527.4994282
GACTTTT705.5445907E-627.4994284
CGACTTT606.1825354E-527.4994283
GAGACAG705.5445907E-627.4994287
AGACAGG651.06578045E-425.3840878
GTCGACT901.3808458E-624.4719981
TATCAAC10550.024.2412012
ATAAGAG701.7624977E-423.5709383
AAGAGAC701.7624977E-423.5709385
TAAGAGA701.7624977E-423.5709384
AGAGACA701.7624977E-423.5709386
GAGCGCC600.001991353722.94251
AGCGCCG600.002004722422.916192
CGCCGCG600.002004722422.916194
GTATAAG1505.456968E-1022.02481
ATCAACG11650.021.7162843
TCAACGC11750.021.5314674
Produced by FastQC (version 0.11.5)