FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798091
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66330.3688912296429936No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT21280.11834773657173081No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT21260.11823650749600548No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT20370.11328681362622915No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC19480.10833711975645281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18650.039.233412
GTGGTAT18800.038.9431271
GCTACGC653.20634E-629.61619420
GTATCAA72650.027.5426581
CGCCGAA904.7240974E-827.49922232
CCGCGTA705.711776E-627.49845511
GCTAGAC759.75436E-625.6673683
GGCGCTA2600.025.38460442
AGAGTAC40500.024.71466311
GGTATCA36550.024.6998021
CCGTGCT801.6085467E-524.06315824
CGCGTAT801.609586E-524.0611512
TATCAAC83900.023.9339392
TATCGTC701.8015198E-423.5707635
TGCCGTA701.8015198E-423.5707638
AGGTCAC701.8018595E-423.57010510
GCGCTAT2900.022.7586143
CGAATTA2200.022.49873715
CAGAGTA45150.022.41291610
GACACTC1605.638867E-1122.3431197
Produced by FastQC (version 0.11.5)