FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649372
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26100.15824204606359268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG2650.036.3229822
GTGGTAT27100.029.1404171
TGGTATC28400.026.9239252
GTATCAA77150.023.1468521
AGAGTAC40650.022.79690711
ACGGGTA2550.021.56667916
TAAACGC4800.021.19921323
CAGAGTA44350.020.95702610
AACGCAG86300.020.329646
TATCAAC87050.020.2851492
AGTACGG46500.019.63263513
GAGTACG46950.019.5621912
ATCAACG90050.019.4213773
TATACGT1702.8521754E-919.4105994
TCAACGC90350.019.2655834
CAACGCA90800.019.1701035
GGTATCA43550.018.638761
CCGACCG909.822086E-418.3322329
GATACCA6700.018.05861711
ATACCAC6600.017.9155912
Produced by FastQC (version 0.11.5)