FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876326
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43910.5010692367908747No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA10190.1162809274174223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA150.00578446154.996869
CTTACGT150.00578446154.9968652
GTGGTAT15900.044.3095971
TGCTACG257.857663E-443.99749419
TGGTATC17650.039.891242
ACGAAGA658.3940904E-833.8442237
GACCTAG957.094059E-1131.8402951
CCACGCA350.00409681831.432163
CCGGCGT350.004100252431.42677728
AGACGTT350.004100252431.4267776
AAAGTCG453.7960862E-430.5538125
CACTGCT4000.030.24827616
TGCTTAA3600.029.78996719
TACTAGG852.709021E-829.1159887
CCGAATT2550.029.11598414
GTATCAA68000.028.8559631
CTGCTTA3800.028.22207518
CCCCGAA2650.028.0172712
CGAATTA2650.028.0172715
TTAAACG3250.027.92148422
Produced by FastQC (version 0.11.5)