FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357703
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56310.41474460909344685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCGG508.3182385E-943.99746726
GCGGTAT300.001918987136.6929321
GTGGTAT14650.034.1882971
GAGTGCG350.004100309731.42792124
AGACTCG350.004101048731.42676211
CTAGTAC553.4917997E-529.9993783
GCCGCCC653.2076987E-629.6136840
CGTAAGG653.2076987E-629.6136825
CCTACGG752.9387775E-729.3413682
CGTATTC852.7084752E-829.11918845
TGGTATC17950.028.3503842
GTATCAA63450.027.5413881
TCCGTTG400.00787159627.50145531
ACGACCG400.00787442627.4994329
GCCCGAT400.00787584127.49841514
TAGTACC606.322661E-527.4974024
CCCGTTG1057.2577677E-926.19186232
TCGGCAT858.7969966E-725.88181729
CCGCCCT858.7969966E-725.88181741
GCTTAAC759.755704E-625.66613420
Produced by FastQC (version 0.11.5)