FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431091
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.23199083775944365No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.17315460721924741No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.1324863338529835No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.11585566536299928No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA14850.10376698616649815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61900.043.605611
GTGGTAT15400.043.058921
TGGTATC15950.041.301862
TATTCGC3450.036.9599239
TATCAAC76200.036.198312
GTCGACT6600.036.066451
GGTATCA30750.035.3878441
CGTATGG250.002789851733.9995784-85
ATCAACG82650.033.6305123
CAACGCA83500.033.542665
TCAACGC85200.032.6239624
ATATTCG4300.032.619288
ACCCTAG2100.032.3861961
AACGCAG93850.030.118376
CGCAGAG96950.028.6282188
TAGTGCA1554.451067E-927.4218799
GCAGAGT102200.027.3239449
ACGCAGA104700.026.671517
CCTTAAT5350.024.6301571
CGACTTT9750.024.4107993
Produced by FastQC (version 0.11.5)