FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431091
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54670.38201623796110795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18750.036.6835861
TCGACGA300.001926044936.6656415
TGGTATC19650.034.4337352
GGGCCGT801.503031E-830.9366366
TTATCGG453.7958522E-430.55576919
GTATCAA79250.029.9949051
AACGAGT705.709453E-627.4992316
TCGTACG606.320034E-527.4992314
TATTCGC2200.026.2492669
ACCGTTC957.976996E-826.05190350
TGGGCCG957.976996E-826.0519035
TATCAAC93200.025.2621022
AGAGTAC39700.025.00559811
CAACGGC550.001226901424.99930240
CGGGCGT550.001226901424.99930225
GCCGTAA550.001226901424.99930249
ATTCGCT2350.024.5737810
TTACCGA801.6085023E-524.06266820
AGACCGT1052.0998232E-723.57076848
GCCGTCC1052.0998232E-723.5707688
Produced by FastQC (version 0.11.5)