FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123267
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98870.46565033978298537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27250.035.7468261
TGGTATC30400.031.8527582
GATCGAC653.2078533E-629.6148247
GTATCAA104050.027.662431
GTGTACG1550.026.6293321
TGCGAAC858.800762E-725.88186646
ACCGAGC651.08952874E-425.3841368
TATCAAC120200.023.7329792
AGAGTAC56050.023.40277111
ACCGACC1203.004061E-822.9162358
CAGAGTA58750.022.51446710
TACGGAC2200.022.5001054
ATCCACG752.8617991E-422.013581
ATCGACC1003.6182682E-621.9995868
CGAACTT903.9982613E-521.38848548
GCGCATT1801.0913936E-1121.38848526
TATGTCG903.9982613E-521.3884856
TATCGGA903.9982613E-521.38848511
ATCAACG133850.021.244033
CAACGCA134250.021.0573335
Produced by FastQC (version 0.11.5)