FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46259
Sequences flagged as poor quality0
Sequence length91
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA6881.48727815127867No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA4540.9814306405240062No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.9209018785533626No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA4050.8755053070753799No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA3420.739315592641432No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT3360.7263451436477225No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.7025659871592556No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.6550076741823212No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGGGG2940.6355520006917573No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA2810.6074493612053871No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA2520.544758857735792No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.5404353747378888No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTGGGGG2410.5209797012473248No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.4128926262997471No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTGGCGG1870.4042456603039408No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA1800.38911346981128No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC1750.3783047623165222No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA1730.3739812793186191No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.36533431332281285No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1610.3480403813312004No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA1450.3134525173479755No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT1400.30264380985321776No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTACGCTG1390.30048206835426616No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.25292375537733197No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.2421150478825742No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAAAAAAAAAAAAAA1120.2421150478825742No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA1070.23130634038781642No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1060.22914459888886485No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC1060.22914459888886485No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA1020.22049763289305863No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1000.21617414989515554No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.2096889253983009No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA870.1880715104087853No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA800.17293931991612443No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATGGGGGGGGGGGG760.1642923539203182No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA760.1642923539203182No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1599688709224151No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGCGG730.15780712942346356No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA720.155645387924512No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATGGGGGGTGGGGG670.14483668042975423No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.14483668042975423No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA670.14483668042975423No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTTGGGG660.14267493893080266No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG650.14051319743185112No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG630.136189714433948No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGTGGGG620.13402797293499644No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA590.12754274843814178No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG580.12538100693919021No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA580.12538100693919021No Hit
CGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCC570.12321926544023865No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAA570.12321926544023865No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.1210575239412871No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC550.11889578244233553No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGGGGGGG550.11889578244233553No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.11889578244233553No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC540.116734040943384No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA540.116734040943384No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG530.11457229944443242No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.11457229944443242No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA530.11457229944443242No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.11241055794548088No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA520.11241055794548088No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTGGCGG510.11024881644652931No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT510.11024881644652931No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCT490.1059253334486262No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCAGCGGGGGGGGGGG490.1059253334486262No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG490.1059253334486262No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10160185045072309No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10160185045072309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1350.053.5185172
TGTATAA1200.053.1250042
TAAGACG401.4364041E-553.1254
GTGGTAT1300.052.307691
GTGTATA1200.049.5833361
CCGCGAG1450.046.8965531
AGCGCCG551.7264683E-646.3636322
GCGAGAT1500.045.3333323
CGCGAGA1500.045.3333322
ATAAGAG1450.043.965525
TAAGAGA1450.043.965526
GAGCGCC603.1423115E-642.5000041
ATAAGAC603.1423115E-642.5000043
GATGTGT409.226769E-442.51
ATGTGTA409.226769E-442.52
CGAGATG1700.040.04
AGAGACA1750.038.8571438
ACGAGCA559.420296E-538.636368
AGACGAG559.420296E-538.636366
GACGAGC559.420296E-538.636367
Produced by FastQC (version 0.11.5)