EGAF00005411393 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411393
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46259
Sequences flagged as poor quality	0
Sequence length	61
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5603	12.112237618625565	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA	461	0.9965628310166671	No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA	173	0.3739812793186191	No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA	170	0.3674960548217644	No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA	155	0.3350699323374911	No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	135	0.29183510235845994	No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA	87	0.1880715104087853	No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA	87	0.1880715104087853	No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA	82	0.17726280291402752	No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA	80	0.17293931991612443	No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.14267493893080266	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.136189714433948	No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.1318662314360449	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG	61	0.1318662314360449	No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG	57	0.12321926544023865	No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA	55	0.11889578244233553	No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.11457229944443242	No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA	52	0.11241055794548088	No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA	48	0.10376359194967466	No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.10160185045072309	No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.10160185045072309	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGAGT	15	0.00575094	54.994595	49
GAGCGCC	55	1.8266292E-8	40.082733	1
AGCGCCG	55	1.8442734E-8	40.039352	2
TGGGAAA	30	0.001907522	36.663063	51
GTGGTAT	205	0.0	34.950188	1
TGGTATC	205	0.0	34.91236	2
GACGGTT	35	0.004061402	31.425482	17
ACGGTTT	35	0.004061402	31.425482	18
GTGTATA	80	1.4084435E-8	31.00149	1
TGTATAA	85	2.5676854E-8	29.146294	2
GCGCCGC	80	4.9579467E-7	27.527056	3
CGGTTTT	40	0.007800293	27.497297	19
TTTATAA	50	6.9336966E-4	27.497297	48
AAGAGAC	95	7.638664E-8	26.050072	7
TAAGAGA	95	7.638664E-8	26.050072	6
CGCCGCG	85	8.418756E-7	25.907818	4
GGTATCA	450	0.0	25.107378	1
AAGGGGG	55	0.0012095333	24.997543	54
CGACTTT	100	1.2420242E-7	24.77435	3
AGAGACA	100	1.2551573E-7	24.747568	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content