FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297199
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36651.2331804615762503No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.9364096110686779No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.8307564964888846No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.6675661761984394No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT16570.5575388880850878No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA11090.37315064990124464No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.3489244580230754No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA9220.3102298459954441No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.2913872523124237No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.2658151608854673No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.25942213802872827No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.25942213802872827No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7090.23856069502252697No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.23553242103775585No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA6630.22308285021147445No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAA6460.21736277712912896No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA6140.20659558073883155No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA6130.20625910585163476No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA5720.19246363547656622No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT5400.1816964390862688No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.17193866735756178No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.1652091696136259No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.1638632700648387No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.1631903202904451No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.1537690234489349No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA4560.1534325485617381No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAA4360.14670305081780222No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA4220.14199240239704708No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.13458995487871764No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.13223463066834007No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.1315616808939465No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3890.13088873111955288No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT3880.13055225623235608No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA3880.13055225623235608No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA3790.12752398224758493No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA3670.12348628360122342No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3570.12012153472925548No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.11978505984205869No Hit
GTGGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTT3530.11877563518046831No Hit
GAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAA3430.11541088630850037No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGGGG3370.1133920369853196No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.11170966254933562No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC3220.10834491367736768No Hit
ATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAA3110.10464368991820296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCT302.2525257E-456.6665574
GTCGCCT250.007785477751.0084841
TACCTCA250.007790659650.99997
CTATCAA452.9185509E-547.230081
ATACCTC651.05716026E-745.769146
TGGTATC17150.042.8716352
TACCGCG505.4422453E-542.4999167
GTGGTAT16850.042.1286661
GTATCAA68400.040.8291551
TCAGATG1153.6379788E-1240.6520963
ACAATAC851.9201252E-839.999923
AATACCG655.5480723E-639.230695
TACGTGC2200.038.636296
AATCAAC1001.882654E-938.2499242
CAATACC1450.038.103374
GAATCAA903.173227E-837.784061
TCACCGC805.394795E-737.1874282
AATACCT805.394795E-737.1874285
GTCAGAT1151.8007995E-1036.956452
TTATCAA709.214633E-636.4346281
Produced by FastQC (version 0.11.5)