FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407217
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.1873911415225939No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.1535655126394863No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.14439848296318195No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA16580.11782120312645455No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT15810.11234941021889304No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.10389300299811614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4300.032.616813
GTATCAA76350.029.3409881
GCGTCAG5050.027.7727284
AACGCAG81000.026.8150256
CGACTTT6050.026.694653
GCAGCGT5550.026.0410881
TATCAAC87350.025.8847942
ATCAACG90950.024.9069483
TTAAACG4800.024.7920722-23
GTCGACT6550.024.6612741
TCAACGC91550.024.604444
CAACGCA92200.024.5231765
TGCGTTG12150.024.4859945
GTGGTAT33500.024.2360441
TCGACTT6750.023.9263152
CGCAGAG92500.023.388538
TGGTATC34500.023.282992
CAGCGTC6250.023.1203772
ACGCAGA95050.022.805787
TCGAGGG22050.022.745759
Produced by FastQC (version 0.11.5)