FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407217
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21160.15036771158961268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG3150.031.42909822
AGAGTAC38300.026.70968211
CAGAGTA40200.025.65250610
TGCGACG550.001226571525.00042222
GTGGTAT32400.024.7984681
TGGTATC33450.023.7631192
GTATCAA87150.023.26951
TATACGT1705.456968E-1222.6466354
AGTACGG45100.022.49957813
GAGTACG45600.022.37348612
ACGGGGC3750.021.99880616
AACGCAG94750.021.244236
TATCAAC96000.021.1726362
CGGTACC650.003239791421.15420245
TAAACGC4950.020.00033623
ATCAACG101650.019.9110873
TCAACGC102250.019.7673574
CGCGACA700.004977117319.6424910
CAACGCA102950.019.6055435
TACGGGG27350.019.5053415
Produced by FastQC (version 0.11.5)