FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113723
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9970.8766916103162948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.330627929266727No Hit
GTGTAAGGGAATTAAAAAGAATCAATCCGAATGTAATGGAATTGAATGGA2810.24709161735093163No Hit
CTATTGCACTCGGGTTGATTGATTCCATTCCATTGCATTCCATTCCATTC2440.2145564221837271No Hit
GAATAGAATCAATACGAATGTAATGGAATGGAATGGAACGGAATGGAATG2440.2145564221837271No Hit
CCCTTACACTCCATTACATTCTATTCCATTCGGGTAGTTTCCATACCATT2400.21103910378727259No Hit
CCCGAGTACAGGGGAATGGAATGCAATGGAATGCAATGGAATGGAATCAT2250.19784915980056803No Hit
GTTTCCATACCATTCCATTCCATTCTATTCGATTTCGTTCCATTCCATTC2140.18817653421031805No Hit
GTACTCGGGTTGATCCCATTCCATTGCATTCCATTCCATTCCATTCCATT2140.18817653421031805No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT2130.18729720461120442RNA PCR Primer, Index 47 (95% over 22bp)
GAATGGAATGGAGTCAACTCGATTGCAATGGAATGCAATGGAATGCAATG2060.18114189741740896No Hit
GTATGGAAACTACCCGAATGGAATAGAATGTAATGGAGTGTAAGGGAATT1990.17498659022361351No Hit
GAATCAACCCGTGTGCAATGGAATGGAAAGGAATGGAATGGAATGGAATG1980.17410726062449988No Hit
GAATTAACCCGAATACAATGGAAAGGAATGGAATGGAATGGAATGTAATG1970.17322793102538625No Hit
GAATTAACCTGAAGAGAATGGAAGGGAATGGAATGGAACGGAACGGAATG1960.17234860142627262No Hit
GGATCAACCCGAGTGAAATGGAATGGAATCGAAAGGAATGGAATGGAATG1940.17058994222804533No Hit
GGTTAATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTGCATTCC1880.1653139646333635No Hit
GAATAGAATGTAATGGAGTGTAAGGGAATTAAAAAGAATCAATCCGAATG1880.1653139646333635No Hit
GCATTCTATTGCACTCGGGTTGATTGATTCCATTCCATTGCATTCCATTC1860.16355530543513624No Hit
ATTCCATTCCATGCCATTCCACTTGGGTTGATTCCCTTCCTTTCCATTCC1850.1626759758360226No Hit
ATTCCATTCCATTCAATTCCGGATGATTCCATTCCATTGCCTTCAATTCC1850.1626759758360226No Hit
GAATGGAATGGAACGGAAAAGAATGCAATGGAACGAAATGGAATGGAATG1850.1626759758360226No Hit
GGAATGGAGTCATCCGGAATGGAACAGAAAGGAATGGAATGGAATCAACC1830.16091731663779535No Hit
GAATTAACCCGAATAGAATGGAATGGAATGGAATGGAATGGAAGTAAATG1820.16003798703868172No Hit
ATTCCATTCCATGCCATTCCACTCGGGTTGATTCCATTCCTTTCCATTCC1720.15124469104754534No Hit
CCCGAATACAATGGAAAGGAATGGAATGGAATGGAATGTAATGGAATGGA1710.1503653614484317No Hit
GTTTCAACCCGAATGGAATGGAAAGGAATGGAATCAACCCGAGTGGAATG1680.14772737265109082No Hit
ATTCCATTCCATTGCCTTCAATTCCATTCCATTCCCCTGCACTCGGGTTG1680.14772737265109082No Hit
GAATGGAACGGAACGGAATGGAATGGAATGGAATGGAATGGAATGGAATG1670.14684804305197718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.14684804305197718No Hit
CCATTGCATTCCATTGCAATCGAGTTGACTCCATTCCATTCCATTCAATT1670.14684804305197718No Hit
GAATGGAATCATCCGGAATTGAATGGAATGGAATGGAGTCAACTCGATTG1670.14684804305197718No Hit
GTACAGGGGAATGGAATGCAATGGAATGCAATGGAATGGAATCATCCCGA1630.14333072465552263No Hit
GAATGGAATGGGTTCAACTCGAATGGAATGGAAAGGAATGGAATCAACCC1620.142451395056409No Hit
GAATGTAATGAACCCGAGGGCAGGGGAATGGAATGGAATGGAATGCAATG1600.14069273585818173No Hit
GAATGGAATCAACCCGAGTGCAGGGGAATGGAATGGAATTGAAGGCAATG1600.14069273585818173No Hit
CCCCTGCACTCGGGTTGATTCCATTCCATTCCATTCCATTCCATTCCATT1600.14069273585818173No Hit
CTATTTGCTTTTCTAACACTTCCTGTCTTTTTTTCCTCATATCTTGTTGT1590.13981340625906807No Hit
GGTTGATTCCATTCCATTCCTTTCTGTTCCATTCCGGATGACTCCATTCC1570.1380547470608408No Hit
GAATGGAACGGAATGGAATGCAATGGAATGGAATGGAATGCAATGGAATG1570.1380547470608408No Hit
GTATCAACGCAGAGTACGGGTGTTCAAGAAGTGCTTAAAAAAAAACAGGA1560.13717541746172718No Hit
ATTCCATTCCATTCCATTGCATTCCATTGCATTCCATTGCAATCGAGTTG1560.13717541746172718No Hit
GAATGGAAAGGAATGGAATGGAATGGAATGGAATGGAATTAACCCGAATA1530.13453742866438628No Hit
GTAATGGAGTGTAAGGGAATTAAAAAGAATCAATCCGAATGTAATGGAAT1510.132778769466159No Hit
GTTTCCATACCATTCCATTCCATTCCACTGCATTCTATTGCACTCGGGTT1500.13189943986704536No Hit
GAATGGAATGCAATGGAATGGAATGGAATGCAATGGAATGGGATCAACCC1490.13102011026793173No Hit
ATTCCATTCCATTCCATTTCATTCCATTCCATTTCGTTCCATTGCATTCT1480.1301407806688181No Hit
GTGCAGGGGAATGGAATGGAATTGAAGGCAATGGAATGGAATCATCCGGA1460.12838212147059083No Hit
GTAATGAACCCGAGGGCAGGGGAATGGAATGGAATGGAATGCAATGGAAT1450.12750279187147717No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAATGCAATGGAATGGGATC1450.12750279187147717No Hit
CTTCCATTCCATTCCATTCCATTCCATGCCATTCCACTCGGGTTGATTCC1440.12662346227236354No Hit
CCCCTGTACTCGGGTTGATCCCATTCCATTGCATTCCATTCCATTCCATT1420.12486480307413628No Hit
CCATTACATTCTATTCCATTCGGGTAGTTTCCATACCATTCCATTCCATT1410.12398547347502265No Hit
ATTCTATTGCACTCGGGTTGATTGATTCCATTCCATTGCATTCCATTCCA1400.12310614387590901No Hit
ATTCGGTACCATTCCATTCCATTCCATTCCATTCCATTCCATGCCATTCC1370.12046815507856809No Hit
CTCCATTTCATTCCATTCCATTCGGGTTGTTTCCATACCATTCCATTCCA1370.12046815507856809No Hit
GAATGGAATGGTTTCAACCCGAATGGAATGGAAAGGAATGGAATCAACCC1370.12046815507856809No Hit
GAATCAACCCGAGTGCAGGGGAATGGAATGGAATTGAAGGCAATGGAATG1360.11958882547945446No Hit
ATGTAATGGAGTGTAAGGGAATTAAAAAGAATCAATCCGAATGTAATGGA1350.11870949588034083No Hit
GAATTGAAGGCAATGGAATGGAATCATCCGGAATTGAATGGAATGGAATG1340.1178301662812272No Hit
GAATGGAATGGAATGGAACGGAAAAGAATGCAATGGAACGAAATGGAATG1340.1178301662812272No Hit
CTTCCATTCCATTCCATTCCATTCCATTCTATTCGGGTTAATTCCATTCC1330.11695083668211356No Hit
GAATGGAACGGAAAAGAATGCAATGGAACGAAATGGAATGGAATGAAATG1330.11695083668211356No Hit
GAATGGAATTGAAGGCAATGGAATGGAATCATCCGGAATTGAATGGAATG1330.11695083668211356No Hit
GAATGGAATGGAATGGAATGGAATGGAATCAACCCGAGTGCAGGGGAATG1310.11519217748388627No Hit
GGTTAATTCCATTCCATTCCATTCCCTTCCATTCCATTCCATTCCATTCC1300.11431284788477264No Hit
GAGTACGGGTGTTCAAGAAGTGCTTAAAAAAAAACAGGAAGCAATGAAGT1290.11343351828565901No Hit
GTGCAATAGAATGCAGTGGAATGGAATGGAATGGTATGGAAACTACCCGA1280.11255418868654538No Hit
AAATGGAATGGAATGAAATGGAATGGAATGGAATGGAATGGAATGGAATG1270.11167485908743174No Hit
GAATGGGATCAACCCGAGTGAAATGGAATGGAATCGAAAGGAATGGAATG1270.11167485908743174No Hit
GAATGGAATGGAATGGTTTCAACCCGAATGGAATGGAAAGGAATGGAATC1270.11167485908743174No Hit
ATTCCATTCCATTCCTTTCTGTTCCATTCCGGATGACTCCATTCCATTGC1250.10991619988920448No Hit
CTCCATTCCATTGCATTCCATTCCATTCCATTCCCCTGCCCTCGGGTTCA1250.10991619988920448No Hit
GAATGGAATCATCCCGAATGGAATGGAATGGAATGGAATGCAATGGAATG1240.10903687029009082No Hit
GAATCAACCCGAGTGGAATGGCATGGAATGGAATGGAATGGAATGGAAGG1220.10727821109186356No Hit
ATTCCATTCCATTCCATTCCATTCCATTGCATTCCATTGCATTCCATTGC1210.10639888149274992No Hit
GAATGGAATGGAATGGATTCAACCCGAATGGAATGGAAAGGAAGGGAATC1210.10639888149274992No Hit
TTTCTGTTCCATTCCGGATGACTCCATTCCATTGCATTCCATTCCATTCC1200.10551955189363629No Hit
GAATGGAATGGAATGGGTTCAACTCGAATGGAATGGAAAGGAATGGAATC1200.10551955189363629No Hit
GAATGGAATCAACCCGTGTGCAATGGAATGGAAAGGAATGGAATGGAATG1200.10551955189363629No Hit
GAATGGAATGGAATGCAATGGAATGGAATCAATCAACCCGAGTGCAATAG1190.10464022229452266No Hit
GTAATGGAATGGAATGGAATGGAATGGAACGGAAAAGAATGCAATGGAAC1190.10464022229452266No Hit
GGTTAATTCCATTCCATTCCATTCCATTCCATTCCTTTCCATTCCGTTCC1190.10464022229452266No Hit
GAATGGAATTAACCCGAATACAATGGAAAGGAATGGAATGGAATGGAATG1190.10464022229452266No Hit
GAATCAACCCGAGTGGAATGCAATGGAATGGAATGGAATGGAATGGGTTC1180.10376089269540903No Hit
GAATGGAATGGAATGGAATGCAATGGAATGGAATCAATCAACCCGAGTGC1180.10376089269540903No Hit
GAATGGAATGGAATGGAATTAACCCGAATACAATGGAAAGGAATGGAATG1180.10376089269540903No Hit
GAATGTAATGGAATGGAATGGAATGGAATGGAACGGAAAAGAATGCAATG1180.10376089269540903No Hit
ATTCCATTCCATTCCATTCCATTGCATTCCATTGCATTCCATTGCAATCG1180.10376089269540903No Hit
GTATTCGGGTTAATTCCATTCCATTCCATTCCATTCCATTCCTTTCCATT1170.10288156309629537No Hit
CCATTACATTCCATTCCATTCCATTCCTTTCCATTGTATTCGGGTTAATT1170.10288156309629537No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT1170.10288156309629537RNA PCR Primer, Index 47 (95% over 24bp)
GCAATGAAGTTACAACAAGATATGAGGAAAAAAAGACAGGAAGTGTTAGA1170.10288156309629537No Hit
ATTCTATTCGGGTTAATTCCATTCCATTCCATTCCATTCCATTCCATTCC1160.10200223349718174No Hit
GAATGGAAAGGAATGGAATCAACCCGAGTGGAATGCAATGGAATGGAATG1150.1011229038980681No Hit
TTTCCATTCCGTTCCATTCAATTCCATTACATTCGGATTGATTCTTTTTA1150.1011229038980681No Hit
GTACCATTCCATTCCATTCCATTCCATTCCATTCCATGCCATTCCACTTG1150.1011229038980681No Hit
ATTCCATTCCATTCCCCTGCACTCGGGTTGATTCCATTCCATTCCATTCC1140.10024357429895447No Hit
CTCCATTACATTCTATTCCATTCGGGTAGTTTCCATACCATTCCATTCCA1140.10024357429895447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)