FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118417
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT16511.394225491272368No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.5480632003850798No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.41294746531325743No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.2685425234552471No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.2533420032596671No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT2870.24236384978508155RNA PCR Primer, Index 47 (95% over 22bp)
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT2190.184939662379557No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.1756504555933692No Hit
CCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAAAAAAGG1950.16467230211878361No Hit
CTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGA1920.16213888208618693No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.15707204202099362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.15453862198839693No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.15116072861160137No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.14271599516961248No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1680.14187152182541357No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGCCGT1540.1300488950066291No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.12751547497403246No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT1510.12751547497403246No Hit
ATCTACTTCTCCCGCCGCCGGGAAAAAAAGGCGGGAGAAGCCCCGGCAGG1480.12498205494143577No Hit
TACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGCTAAGC1440.12160416156464021No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12160416156464021No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.11991521487624242No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1410.11907074153204354No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGT1410.11907074153204354No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGG1410.11907074153204354No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT1380.11653732149944687RNA PCR Primer, Index 47 (95% over 24bp)
GCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCT1370.11569284815524797No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.11062600809005464No Hit
GTACATTGAGTGCCCTGGTATTATTGACAAAAGAGAATTTGACAACATTG1280.10809258805745796No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATGCGCA1220.10302574799226462No Hit
CAGCTAAGGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGC1200.10133680130386684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG150.00102580585.030424
CACGGGC250.007768091351.0182537
TACACGG250.007768091351.0182535
ATATTAC250.007768091351.0182533
ATTACAC354.8008616E-448.5888143
GTCGACT1550.046.6295851
GTATCAA9800.045.5520131
TCGACTT1600.045.172412
TGGTATC1900.044.752852
AAGGTTG409.269164E-442.515215
TAAGGTT409.269164E-442.515214
CTGCTTA209.307359E-442.49725318-19
TGCTTAA209.307359E-442.49725318-19
GTGGTAT2050.041.4782521
CGACTTT1700.040.0143173
TTAGACC655.5061737E-639.244814
TATCAAC11550.039.0182882
CTAAGGT450.001654115437.7912983
GACAGTT709.155805E-636.441617
CCGTGGT651.6370905E-1135.95921746-47
Produced by FastQC (version 0.11.5)