FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118417
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69185.842066595167923No Hit
CTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAA1970.1663612488071814No Hit
CCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAAAAAAGGCGGGAGAAGCC1580.13342678838342467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.12329310825303799No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTCC150.00577155454.9983149
ACACAGG150.00577155454.99831417
CTCCAGT150.00577155454.99831411
CACCTGG150.00577155454.99831421
TCCAGTG150.00577155454.99831412
CAGATGT150.00577155454.9983147
AGATGTC150.00577155454.9983148
TGTCCAG150.00577155454.99831411
ACAGGTG202.6044314E-454.9983110
CACGGGC303.4727756E-545.831937
ACGGGCC303.4727756E-545.831938
GGGCCTT303.4727756E-545.8319310
TACACGG303.4727756E-545.831935
CACAGGG257.829052E-443.9986518
TCCTCTC358.6007916E-539.3011052
ACACGGG358.6222426E-539.284516
CGGGCCT358.6222426E-539.284519
TTCCATC300.001918953436.66554321
TATTCCA300.001918953436.66554319
TCTCCCA300.001918953436.6655435
Produced by FastQC (version 0.11.5)