FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219776
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46720.38302114486594263No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.29390642216275775No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.2203683299228711No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT25950.21274397922241461No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC24640.20200430242929848No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24000.19675743743113489No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA23780.19495382758801616No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC21870.17929521485912167No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA19620.16084920509995276No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA19120.15675009182013747No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT18920.15511044650821135No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA18260.14969961697885512No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT18210.1492897056508736No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA16540.13559866729629047No Hit
CATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTAA14810.1214157353481295No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT13710.11239768613253581No Hit
GTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCATTGC13460.11034812949262816No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAG13420.11002020043024292No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.10854451964950942No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT12650.10370756597932736No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.10133008027703447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA200.003223635363.7494749
CACGTTC250.007791565351.0079461
GACCGTC452.926948E-547.2218367
GGACCGT801.1290467E-842.5013926
GTATCAA90500.040.815751
GTGGTAT21350.039.818851
ACCGTCC559.562643E-538.6360478
CAGTACG1550.038.3867849
TATCAAC103250.035.9769672
TGGTATC24350.035.257832
TATACGT1008.064126E-834.0011144
ATTGTAC901.3598547E-633.056643
ACAGTAC1850.032.1618968
ATCAACG117400.031.966553
GGTATCA44800.031.6902051
CAACGCA120350.031.1476765
TCAACGC121250.030.8814264
AACGCAG121850.030.48526
GTCGACT9950.029.0497511
TGGACCG1203.9888073E-728.3342635
Produced by FastQC (version 0.11.5)