FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219776
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111910.9174635342882628No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT18270.14978159924445145No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT18120.14855186526050684No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT17090.1401076919040873No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA16930.1387959756545464No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT16910.1386320111233538No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC15960.1308436958917047No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT14360.11772653339629571No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAG13840.11346345558528778No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTA13530.11092200535180231No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAAC12770.10469135316648302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA202.6137213E-454.9998789
GACGGTC202.6142507E-454.9976237
GGACGGT303.4922312E-545.8313526
CGGACCT257.8583433E-443.99809622
GCGGTAT403.9268325E-641.270211
GTGGTAT25550.036.8282131
CGTGCAC300.001925690736.66658410
TCCGATC1300.035.96293331
CATCGGT1300.035.95998825
TCGGTCC1300.035.95998827
CCGATCA1350.034.63097432
CGGTCCG1350.034.6281328
CCGTGCG401.902097E-434.37351616
AGCGACC658.3975465E-833.84469217
GTAACGC658.3975465E-833.84469224
GTCATCG1400.033.39141523
ATCGGTC1400.033.39141526
TAGGTCG501.8206243E-532.9985735
TGGTATC28250.032.8079262
ACGGTCC350.00409977731.4285018
Produced by FastQC (version 0.11.5)