FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991
Sequences flagged as poor quality0
Sequence length91
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT6387.983981979727194No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT1051.3139782255036916RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGT750.9385558753597798No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGCCGT670.8384432486547365No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATGCGCA630.7883869353022149No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGG620.7758728569640846No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT520.6507320735827806No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT510.6382179952446503RNA PCR Primer, Index 47 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGAGCTCGTATGCCGT470.5881616818921287No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC430.5381053685396071No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCGT350.4379927418345639No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA340.4254786634964335No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATATCGTATGCCGT300.3754223501439119No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG300.3754223501439119No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGGCGT290.3629082718057815No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC260.3253660367913903No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA250.3128519584532599No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGGATGCCGT210.26279564510073833No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA180.22525341008634714No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA180.22525341008634714No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.21273933174821674No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA170.21273933174821674No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTTTGCCGT160.20022525341008632No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA150.18771117507195595No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACATGCGCAGAT150.18771117507195595No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATGCGCAGA140.17519709673382555No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCG140.17519709673382555RNA PCR Primer, Index 47 (95% over 23bp)
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.17519709673382555No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT130.16268301839569516No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT130.16268301839569516RNA PCR Primer, Index 47 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTGTT130.16268301839569516No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG120.15016894005756476No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGG120.15016894005756476No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACAT120.15016894005756476No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG120.15016894005756476TruSeq Adapter, Index 9 (95% over 24bp)
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACATGC120.15016894005756476No Hit
GCCCTATCCACCACGGGTATACCGGTTAGGTATGAAGTGGTCATGTACTA110.13765486171943436No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTGGT110.13765486171943436No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATATCGTATGCCGTCTT100.12514078338130397No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATTCCGT100.12514078338130397No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.12514078338130397No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACA100.12514078338130397No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGGCGT90.11262670504317357No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGCCGTCTT90.11262670504317357No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGCTCGCGTATGCCGT90.11262670504317357No Hit
CATATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT90.11262670504317357No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11262670504317357No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGAGCGCGTATGCCGT90.11262670504317357No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTGTGCCGT80.10011262670504316No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGG80.10011262670504316No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCCT80.10011262670504316No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGTCTT80.10011262670504316No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCG80.10011262670504316No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTGT80.10011262670504316Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGGCGG80.10011262670504316No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGGATGCCGG80.10011262670504316No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGAGCTCGTATGCCGTCTT80.10011262670504316No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAAGCCGT80.10011262670504316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA3500.038.8571432
CTTATAC3550.038.309861
TATACAC3650.037.2602733
TACACAT4250.032.0000045
ATACACA4300.031.6279054
TGCCGTG300.006608647328.33333244-45
CGTGGAA300.006608647328.33333256-57
TGGGGGT408.242082E-426.562552-53
CACATCT4850.026.288667
ACACATC4850.026.288666
ACATCTC4900.026.0204078
GTGGAAA758.604911E-825.558-59
CATCTCC5100.024.9999989
TTCTGCT1059.543328E-820.23809452-53
CGTCTTC1603.6379788E-1219.92187548-49
CCGTCTT1950.019.61538546-47
CAGATCT2600.019.61538534-35
CTTCTGC1302.0008883E-919.61538550-51
ATGCCGT2550.019.16666644-45
TCTGCTT1001.3779481E-619.12552-53
Produced by FastQC (version 0.11.5)