FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991
Sequences flagged as poor quality0
Sequence length61
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275434.46377174321111No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA200.25028156676260793No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA110.13765486171943436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGG80.10011262670504316No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTT350.00386169131.4285725
TTGGGGT350.00386169131.4285724
ATTGGGG400.007419586627.53
GGATTGG400.007419586627.51
GATTGGG400.007419586627.52
Produced by FastQC (version 0.11.5)