FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439924
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.13931290818126513No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.10917242854483987No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.10257485811751176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13900.043.4274861
GTATCAA52700.038.880121
TGGTATC15650.038.2917672
AGCGTCA3150.033.7310683
TATCAAC62650.033.308962
ATCAACG65600.032.0702253
CAACGCA66650.031.8200655
TCAACGC66400.031.7478454
GGTATCA28100.031.617761
GCGTCAG3600.029.5146854
AACGCAG72500.029.253536
TATACGG1353.5326593E-828.3340992
ACGCAGA84500.025.1486077
CGCAGAG88600.023.8889068
GCAGAGT89950.023.4358739
TATACGT2002.6084308E-923.375634
TCGAGGG18500.023.203339
GTATACG1652.4600376E-723.1872751
GTCGACT4450.021.0161821
ACGGGCG1857.4161835E-720.6762335
Produced by FastQC (version 0.11.5)