FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439924
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52250.362866373503046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16250.036.419821
TGGTATC16450.035.783572
GTATCAA66700.027.980571
AGAGTAC40100.024.68642411
TATCAAC76250.024.2778952
CGAACTA801.6091019E-524.06152729
CAGAGTA42150.023.35530510
AGTCGTA600.002036256722.91574129
TAGTTCG600.00203666822.9149467
ATCAACG80700.022.7964763
GGTATCA33100.022.7032171
TCAACGC81500.022.4707034
CAACGCA81800.022.4219115
TATACGT1505.984475E-1021.9983485
AGTACGG43400.021.98567813
GAGTACG45200.021.65766712
AACGCAG86050.021.4742726
ATACGTG1559.0221874E-1021.2887216
TACGGGT3300.020.83176815
AACGGCC804.4442384E-420.62416637
Produced by FastQC (version 0.11.5)