FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704395
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56860.8072175412943022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG150.005783157454.99886725
ATCTCGC150.005783157454.99886740
GGCCACT150.005783157454.99886750
ATCTTGC403.9133392E-641.287251
CGAAGTC753.6379788E-1240.332537
GTGGTAT3200.039.566951
ACGAGCA358.6653585E-539.28498
TGCTCTG504.1221756E-738.4992035
ACGAAGT809.094947E-1237.81171836
TGGCACC300.001925312836.6659120
TTGCTCT558.6949694E-734.999284
CTGTGGC558.6949694E-734.999289
AAGACGA401.9010308E-434.374295
GACGAGC401.9010308E-434.374297
ATCGCCG658.38736E-833.84545522
CACGAAG903.8198777E-1133.61041635
TCTTAGC1003.6379788E-1232.99931723
GTCCGAG501.819257E-532.99931722
CTTGCTC601.7152888E-632.084953
TTCTTAG1057.2759576E-1231.42792122
Produced by FastQC (version 0.11.5)